X-ray diffraction
1.8Å resolution

Crystal Structure of a Novel Engineered Retroaldolase: RA-61


Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Endo-1,4-beta-xylanase Chain: B
Molecule details ›
Chain: B
Length: 198 amino acids
Theoretical weight: 21.92 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
  • Canonical: Q8GMV7 (Residues: 41-237; Coverage: 65%)
Gene names: FHX40_0105, xyn11A
Sequence domains: Glycosyl hydrolases family 11
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 39.19Å b: 66.06Å c: 66.07Å
α: 90° β: 90° γ: 90°
R R work R free
0.206 0.203 0.248
Expression system: Escherichia coli