3b4t

X-ray diffraction
2.1Å resolution

Crystal structure of Mycobacterium tuberculosis RNase PH, the Mycobacterium tuberculosis Structural Genomics Consortium target Rv1340

Released:
Entry authors: Antczak AJ, Berger JM, Lekin T, Segelke BW, Mycobacterium Tuberculosis Structural Proteomics Project (XMTB), TB Structural Genomics Consortium (TBSGC)

Function and Biology Details

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease PH Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 262 amino acids
Theoretical weight: 27.63 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGZ7 (Residues: 2-259; Coverage: 100%)
Gene names: MTCY02B10.04, MTCY130.25, Rv1340, rph, rphA
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 73.529Å b: 152.406Å c: 83.512Å
α: 90° β: 109.46° γ: 90°
R-values:
R R work R free
0.194 0.194 0.229
Expression system: Escherichia coli