› 3b48

X-ray diffraction
2.21Å resolution

Crystal structure of unknown function protein EF1359

Released:
DOI: 10.2210/pdb3b48/pdb
PDB 3b48 coloured by chain and viewed from the front.
PDB 3b48 coloured by chain and viewed from the side.
PDB 3b48 coloured by chain and viewed from the top.
Source organism: Enterococcus faecalis V583
Entry authors: Chang C, Li H, Moy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + glycerone = pyruvate + glycerone phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
phosphoenolpyruvate--glycerone phosphotransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 135 amino acids
Theoretical weight: 14.75 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli
UniProt:
  • Canonical: Q835L8 (Residues: 1-132; Coverage: 100%)
Gene name: EF_1359
Sequence domains: PTS system fructose IIA component
Structure domains: Phosphotransferase system, mannose-type IIA component

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 36 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 60.08Å b: 102.112Å c: 60.454Å
α: 90° β: 96.57° γ: 90°
R-values:
R R work R free
0.203 0.201 0.246
Expression system: Escherichia coli

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