› 3b1y

X-ray diffraction
2.5Å resolution

Crystal structure of an S. thermophilus NFeoB T35A mutant bound to GDP

Released:
DOI: 10.2210/pdb3b1y/pdb
PDB entry 3b1y coloured by chain, front view.
PDB entry 3b1y coloured by chain, side view.
PDB entry 3b1y coloured by chain, top view.
Source organism: Streptococcus thermophilus LMG 18311
Primary publication:
A suite of Switch I and Switch II mutant structures from the G-protein domain of FeoB.
Ash MR, Maher MJ, Guss JM, Jormakka M
Acta Crystallogr D Biol Crystallogr 67 973-80 (2011)
PMID: 22101824

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177602 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferrous iron transport protein B Chain: A
Molecule details ›
Chain: A
Length: 272 amino acids
Theoretical weight: 30.17 KDa
Source organism: Streptococcus thermophilus LMG 18311
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5M586 (Residues: 1-270; Coverage: 38%)
Gene names: feoB, stu0608
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GNH 1 x GNH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P41212
Unit cell:
a: 42.8Å b: 42.8Å c: 281.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.22 0.26
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Bacterial ferrous iron transport: the Feo system.
Lau et al. (2016)
1 more

1 mention without citation

Ins and Outs: Recent Advancements in Membrane Protein-Mediated Prokaryotic Ferrous Iron Transport.
Brown et al. (2021)