3b1n

X-ray diffraction
1.55Å resolution

Structure of Burkholderia thailandensis nucleoside kinase (BthNK) in complex with ADP-mizoribine

Released:

Function and Biology Details

Reaction catalysed:
Diphosphate + a purine nucleoside = phosphate + a purine mononucleotide
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PfkB domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 326 amino acids
Theoretical weight: 35.75 KDa
Source organism: Burkholderia thailandensis E264
Expression system: Rhodococcus erythropolis
UniProt:
  • Canonical: Q2SZE4 (Residues: 1-312; Coverage: 100%)
Gene name: BTH_I1158
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2221
Unit cell:
a: 44.583Å b: 164.654Å c: 182.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.198
Expression system: Rhodococcus erythropolis