› 3b1n

X-ray diffraction
1.55Å resolution

Structure of Burkholderia thailandensis nucleoside kinase (BthNK) in complex with ADP-mizoribine

Released:
DOI: 10.2210/pdb3b1n/pdb
PDB entry 3b1n coloured by chain, front view.
PDB entry 3b1n coloured by chain, side view.
PDB entry 3b1n coloured by chain, top view.
Source organism: Burkholderia thailandensis E264
Primary publication:
Structures of Burkholderia thailandensis nucleoside kinase: implications for the catalytic mechanism and nucleoside selectivity.
Yasutake Y, Ota H, Hino E, Sakasegawa S, Tamura T
Acta Crystallogr D Biol Crystallogr 67 945-56 (2011)
PMID: 22101821

Function and Biology Details

Reaction catalysed: 
Diphosphate + a purine nucleoside = phosphate + a purine mononucleotide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173982 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbohydrate kinase PfkB domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 326 amino acids
Theoretical weight: 35.75 KDa
Source organism: Burkholderia thailandensis E264
Expression system: Rhodococcus erythropolis
UniProt:
  • Canonical: Q2SZE4 (Residues: 1-312; Coverage: 100%)
Gene name: BTH_I1158
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

4 bound ligands:
Ligand MZR 2 x MZR
Ligand ADP 2 x ADP
Ligand NA 2 x NA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2221
Unit cell:
a: 44.583Å b: 164.654Å c: 182.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.198
Expression system: Rhodococcus erythropolis

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Citations

2 mentions without citation

A broad specificity nucleoside kinase from Thermoplasma acidophilum.
Elkin et al. (2013)
1 more