› 3b0b

X-ray diffraction
2.15Å resolution

Crystal structure of the chicken CENP-S/CENP-X complex

Released:
DOI: 10.2210/pdb3b0b/pdb
PDB 3b0b coloured by chain and viewed from the front.
PDB 3b0b coloured by chain and viewed from the side.
PDB 3b0b coloured by chain and viewed from the top.
Source organism: Gallus gallus
Primary publication:
CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold.
Nishino T, Takeuchi K, Gascoigne KE, Suzuki A, Hori T, Oyama T, Morikawa K, Cheeseman IM, Fukagawa T
Cell 148 487-501 (2012)
PMID: 22304917

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero dimer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Centromere protein S Chains: A, B
Molecule details ›
Chains: A, B
Length: 107 amino acids
Theoretical weight: 12.07 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: E1BSW7 (Residues: 2-106; Coverage: 76%)
Gene names: APITD1, CENPS
Sequence domains: CENP-S protein
Structure domains: Histone, subunit A
Centromere protein X Chains: C, D
Molecule details ›
Chains: C, D
Length: 81 amino acids
Theoretical weight: 9.41 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DJH7 (Residues: 1-80; Coverage: 100%)
Gene names: CENPX, STRA13
Sequence domains: CENP-S associating Centromere protein X
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1, SPRING-8 BEAMLINE BL44XU
Spacegroup: P61
Unit cell:
a: 48.741Å b: 48.741Å c: 345.633Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.186 0.235
Expression system: Escherichia coli

Quick links

Citations

44 review citations

Kinetochore Architecture Employs Diverse Linker Strategies Across Evolution.
Sridhar et al. (2022)
43 more

2 mentions without citation

CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold.
Nishino et al. (2012)
1 more