› 3azx

X-ray diffraction
1.8Å resolution

Crystal structure of the laminarinase catalytic domain from Thermotoga maritima MSB8

Released:
DOI: 10.2210/pdb3azx/pdb
PDB 3azx coloured by chain and viewed from the front.
PDB 3azx coloured by chain and viewed from the side.
PDB 3azx coloured by chain and viewed from the top.
Source organism: Thermotoga maritima MSB8
Primary publication:
Crystal structures of the laminarinase catalytic domain from Thermotoga maritima MSB8 in complex with inhibitors: essential residues for ��-1,3- and ��-1,4-glucan selection.
Jeng WY, Wang NC, Lin CT, Shyur LF, Wang AH
J. Biol. Chem. 286 45030-40 (2011)
PMID: 22065588

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GH16 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 272 amino acids
Theoretical weight: 31.24 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WXN1 (Residues: 204-466; Coverage: 41%)
Gene name: TM_0024
Sequence domains: Glycosyl hydrolases family 16
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: C2
Unit cell:
a: 100.567Å b: 56.152Å c: 126.431Å
α: 90° β: 105.26° γ: 90°
R-values:
R R work R free
0.201 0.198 0.248
Expression system: Escherichia coli

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Citations

16 citation in other articles

The Recognition of Identical Ligands by Unrelated Proteins.
Barelier et al. (2015)
15 more

5 mentions without citation

The β-glucanase ZgLamA from Zobellia galactanivorans evolved a bent active site adapted for efficient degradation of algal laminarin.
Labourel et al. (2014)
4 more