3ayr

X-ray diffraction
2Å resolution

GH5 endoglucanase EglA from a ruminal fungus

Released:
Source organism: Piromyces rhizinflatus
Primary publication:
Substrate binding of a GH5 endoglucanase from the ruminal fungus Piromyces rhizinflata.
Acta Crystallogr Sect F Struct Biol Cryst Commun 67 1189-94 (2011)
PMID: 22102024

Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellulase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 376 amino acids
Theoretical weight: 43.38 KDa
Source organism: Piromyces rhizinflatus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9URH5 (Residues: 1-362; Coverage: 100%)
Gene name: eglA
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P6122
Unit cell:
a: 83.396Å b: 83.396Å c: 225.085Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.17 0.216
Expression system: Escherichia coli