› 3av3

X-ray diffraction
1.7Å resolution

Crystal structure of glycinamide ribonucleotide transformylase 1 from Geobacillus kaustophilus

Released:
DOI: 10.2210/pdb3av3/pdb
PDB entry 3av3 coloured by chain, front view.
PDB entry 3av3 coloured by chain, side view.
PDB entry 3av3 coloured by chain, top view.
Source organism: Geobacillus kaustophilus HTA426
Primary publication:
Structures and reaction mechanisms of the two related enzymes, PurN and PurU.
Sampei G, Kanagawa M, Baba S, Shimasaki T, Taka H, Mitsui S, Fujiwara S, Yanagida Y, Kusano M, Suzuki S, Terao K, Kawai H, Fukai Y, Nakagawa N, Ebihara A, Kuramitsu S, Yokoyama S, Kawai G
J Biochem 154 569-79 (2013)
PMID: 24108189

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177420 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosylglycinamide formyltransferase Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 23.47 KDa
Source organism: Geobacillus kaustophilus HTA426
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5L3C9 (Residues: 1-210; Coverage: 100%)
Gene names: GK0266, purN
Sequence domains: Formyl transferase
Structure domains: Formyl transferase, N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: C2221
Unit cell:
a: 51.861Å b: 117.408Å c: 67.251Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.214
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

The In Vitro Redundant Enzymes PurN and PurT Are Both Essential for Systemic Infection of Mice in Salmonella enterica Serovar Typhimurium.
Jelsbak et al. (2016)
2 more

1 mention without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)