› 3aql

X-ray diffraction
3Å resolution

Structure of bacterial protein (apo form II)

Released:
DOI: 10.2210/pdb3aql/pdb
PDB entry 3aql coloured by chain, front view.
PDB entry 3aql coloured by chain, side view.
PDB entry 3aql coloured by chain, top view.
Source organism: Escherichia coli DH1
Primary publication:
Mechanism for the alteration of the substrate specificities of template-independent RNA polymerases.
Toh Y, Takeshita D, Nagaike T, Numata T, Tomita K
Structure 19 232-43 (2011)
PMID: 21300291

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(A) polymerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 415 amino acids
Theoretical weight: 48.02 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli DH1
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P42212
Unit cell:
a: 133.012Å b: 133.012Å c: 176.845Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.237 0.251
Expression system: Escherichia coli DH1

Quick links

Citations

4 review citations

The CCA-adding enzyme: A central scrutinizer in tRNA quality control.
Betat et al. (2015)
3 more

1 mention without citation

NMR reveals structural rearrangements associated to substrate insertion in nucleotide-adding enzymes.
Mohanty et al. (2016)