X-ray diffraction
2.4Å resolution

Crystal structure of human tyrosylprotein sulfotransferase-2 complexed with PAP,C4 peptide, and phosphate ion

Source organism: Homo sapiens
Entry authors: Teramoto T, Fujikawa Y, Kawaguchi Y, Kurogi K, Soejima M, Adachi R, Nakanishi Y, Mishiro-Sato E, Liu M-C, Sakakibara Y, Suiko M, Kimura M, Kakuta Y

Function and Biology Details

Reaction catalysed:
3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein-tyrosine sulfotransferase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 337 amino acids
Theoretical weight: 37.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: O60704 (Residues: 43-359; Coverage: 84%)
Gene name: TPST2
Sequence domains: Sulfotransferase family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
C4 peptide Chains: S, T
Molecule details ›
Chains: S, T
Length: 9 amino acids
Theoretical weight: 1.21 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: R32
Unit cell:
a: 138.379Å b: 138.379Å c: 228.547Å
α: 90° β: 90° γ: 120°
R R work R free
0.209 0.207 0.249
Expression systems:
  • Escherichia coli
  • Not provided