› 3amr

X-ray diffraction
1.25Å resolution

Crystal Structures of Bacillus subtilis Alkaline Phytase in Complex with Ca2+, Co2+, Ni2+, Mg2+ and myo-Inositol Hexasulfate

Released:
DOI: 10.2210/pdb3amr/pdb
PDB entry 3amr coloured by chain, front view.
PDB entry 3amr coloured by chain, side view.
PDB entry 3amr coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structures of Bacillus alkaline phytase in complex with divalent metal ions and inositol hexasulfate.
Zeng YF, Ko TP, Lai HL, Cheng YS, Wu TH, Ma Y, Chen CC, Yang CS, Cheng KJ, Huang CH, Guo RT, Liu JR
J Mol Biol 409 214-24 (2011)
PMID: 21463636

Function and Biology Details

Reaction catalysed: 
Myo-inositol hexakisphosphate + H(2)O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128267 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-phytase Chain: A
Molecule details ›
Chain: A
Length: 355 amino acids
Theoretical weight: 39.13 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O31097 (Residues: 29-383; Coverage: 99%)
Gene names: phyB13, phyC
Sequence domains: Phytase
Structure domains: TolB, C-terminal domain

Ligands and Environments

2 bound ligands:
Ligand IHS 1 x IHS
Ligand CA 11 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P21212
Unit cell:
a: 90.129Å b: 73.463Å c: 52.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.139 0.137 0.178
Expression system: Escherichia coli

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Citations

1 review citation

β-Propeller phytases: Diversity, catalytic attributes, current developments and potential biotechnological applications.
Kumar et al. (2017)
14 other articles

5 mentions without citation

Research status of Bacillus phytase.
Zhao et al. (2021)
4 more