› 3ajq

X-ray diffraction
1.58Å resolution

Crystal structure of human H ferritin E140Q mutant

Released:
DOI: 10.2210/pdb3ajq/pdb
PDB entry 3ajq coloured by chain, front view.
PDB entry 3ajq coloured by chain, side view.
PDB entry 3ajq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The universal mechanism for iron translocation to the ferroxidase site in ferritin, which is mediated by the well conserved transit site.
Masuda T, Goto F, Yoshihara T, Mikami B
Biochem Biophys Res Commun 400 94-9 (2010)
PMID: 20705053

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136244 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin heavy chain Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 21.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02794 (Residues: 2-183; Coverage: 100%)
Gene names: FTH, FTH1, FTHL6, OK/SW-cl.84, PIG15
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

1 bound ligand:
Ligand MG 11 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: F432
Unit cell:
a: 182.354Å b: 182.354Å c: 182.354Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.175 0.185
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Iron, Ferritin, Hereditary Ferritinopathy, and Neurodegeneration.
Muhoberac et al. (2019)
2 more