3aiz

X-ray diffraction
2.8Å resolution

Crystal structure of PCNA2-PCNA3 complex from Sulfolobus tokodaii (P21212)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-188828 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA polymerase sliding clamp 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 27.57 KDa
Source organism: Sulfurisphaera tokodaii str. 7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q975M2 (Residues: 1-248; Coverage: 100%)
Gene names: STK_03970, pcn2, pcnB
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen
DNA polymerase sliding clamp 3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 246 amino acids
Theoretical weight: 27.46 KDa
Source organism: Sulfurisphaera tokodaii str. 7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q973F5 (Residues: 1-246; Coverage: 100%)
Gene names: STK_09440, pcn3, pcnC
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21212
Unit cell:
a: 91.11Å b: 160.57Å c: 116.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.223 0.256
Expression system: Escherichia coli BL21(DE3)