› 3ai0

X-ray diffraction
1.4Å resolution

Crystal structure of beta-glucosidase from termite Neotermes koshunensis in complex with para-nitrophenyl-beta-D-glucopyranoside

Released:
DOI: 10.2210/pdb3ai0/pdb
PDB entry 3ai0 coloured by chain, front view.
PDB entry 3ai0 coloured by chain, side view.
PDB entry 3ai0 coloured by chain, top view.
Source organism: Neotermes koshunensis
Primary publication:
Structural and functional analysis of three β-glucosidases from bacterium Clostridium cellulovorans, fungus Trichoderma reesei and termite Neotermes koshunensis.
Jeng WY, Wang NC, Lin MH, Lin CT, Liaw YC, Chang WJ, Liu CI, Liang PH, Wang AH
J Struct Biol 173 46-56 (2011)
PMID: 20682343

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186121 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucosidase Chain: A
Molecule details ›
Chain: A
Length: 487 amino acids
Theoretical weight: 55.61 KDa
Source organism: Neotermes koshunensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8T0W7 (Residues: 21-498; Coverage: 100%)
Gene name: NkBG
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand PNW 1 x PNW
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: C2
Unit cell:
a: 93.089Å b: 68.755Å c: 75.746Å
α: 90° β: 95.68° γ: 90°
R-values:
R R work R free
0.123 0.122 0.153
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Bioproducts From Euglena gracilis: Synthesis and Applications.
Gissibl et al. (2019)
7 more

7 mentions without citation

A mechanism of glucose tolerance and stimulation of GH1 β-glucosidases.
Yang et al. (2015)
6 more