› 3ahj

X-ray diffraction
2.1Å resolution

H553A mutant of Phosphoketolase from Bifidobacterium Breve

Released:
DOI: 10.2210/pdb3ahj/pdb
PDB entry 3ahj coloured by chain, front view.
PDB entry 3ahj coloured by chain, side view.
PDB entry 3ahj coloured by chain, top view.
Source organism: Bifidobacterium breve
Primary publication:
Crystal structures of phosphoketolase: thiamine diphosphate-dependent dehydration mechanism.
Suzuki R, Katayama T, Kim BJ, Wakagi T, Shoun H, Ashida H, Yamamoto K, Fushinobu S
J Biol Chem 285 34279-87 (2010)
PMID: 20739284

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable phosphoketolase Chain: A
Molecule details ›
Chain: A
Length: 845 amino acids
Theoretical weight: 94.9 KDa
Source organism: Bifidobacterium breve
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D6PAH1 (Residues: 1-825; Coverage: 100%)
Gene name: xfp
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand TPP 1 x TPP
Ligand EDO 11 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: I422
Unit cell:
a: 175.077Å b: 175.077Å c: 163.996Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.158 0.191
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

CC bond formation using ThDP-dependent lyases.
Müller et al. (2013)
2 more

2 mentions without citation

SIFTS: updated Structure Integration with Function, Taxonomy and Sequences resource allows 40-fold increase in coverage of structure-based annotations for proteins.
Dana et al. (2019)
1 more