› 3af5

X-ray diffraction
2.6Å resolution

The crystal structure of an archaeal CPSF subunit, PH1404 from Pyrococcus horikoshii

Released:
DOI: 10.2210/pdb3af5/pdb
PDB entry 3af5 coloured by chain, front view.
PDB entry 3af5 coloured by chain, side view.
PDB entry 3af5 coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Crystal structure of an archaeal cleavage and polyadenylation specificity factor subunit from Pyrococcus horikoshii.
Nishida Y, Ishikawa H, Baba S, Nakagawa N, Kuramitsu S, Masui R
Proteins 78 2395-8 (2010)
PMID: 20544974

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129299 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription termination factor FttA Chain: A
Molecule details ›
Chain: A
Length: 651 amino acids
Theoretical weight: 74.06 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O50112 (Residues: 1-651; Coverage: 100%)
Gene names: PH1404, fttA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 6 x SO4
Ligand ACY 4 x ACY
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P43212
Unit cell:
a: 86.2Å b: 86.2Å c: 238.287Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

KH-RNA interactions: back in the groove.
Nicastro et al. (2015)
5 more

4 mentions without citation

Structural biology of poly(A) site definition.
Yang et al. (2011)
3 more