3ach

X-ray diffraction
1.4Å resolution

Crystal Structure of Carbohydrate-Binding Module Family 28 from Clostridium josui Cel5A in complex with cellotetraose

Released:

Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-1,4-endoglucanase Chain: A
Molecule details ›
Chain: A
Length: 203 amino acids
Theoretical weight: 22.39 KDa
Source organism: Ruminiclostridium josui
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59290 (Residues: 560-752; Coverage: 21%)
Gene name: celA
Structure domains: Galactose-binding domain-like

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 39.576Å b: 63.9Å c: 75.619Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.198
Expression system: Escherichia coli