› 3aaj

X-ray diffraction
2.4Å resolution

Crystal structure of Ca2+-bound form of des3-23ALG-2deltaGF122

Released:
DOI: 10.2210/pdb3aaj/pdb
PDB entry 3aaj coloured by chain, front view.
PDB entry 3aaj coloured by chain, side view.
PDB entry 3aaj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for defect in Alix-binding by alternatively spliced isoform of ALG-2 (ALG-2DeltaGF122) and structural roles of F122 in target recognition.
Inuzuka T, Suzuki H, Kawasaki M, Shibata H, Wakatsuki S, Maki M
BMC Struct Biol 10 25 (2010)
PMID: 20691033

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130902 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Programmed cell death protein 6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 167 amino acids
Theoretical weight: 19.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75340 (Residues: 24-191; Coverage: 87%)
Gene names: ALG2, PDCD6
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand CA 6 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21
Unit cell:
a: 42.58Å b: 78.94Å c: 60.333Å
α: 90° β: 104.58° γ: 90°
R-values:
R R work R free
0.2 0.196 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Structure and function of ALG-2, a penta-EF-hand calcium-dependent adaptor protein.
Maki et al. (2011)
1 more

1 mention without citation

Molecular basis for defect in Alix-binding by alternatively spliced isoform of ALG-2 (ALG-2DeltaGF122) and structural roles of F122 in target recognition.
Inuzuka et al. (2010)