3aaf

X-ray diffraction
1.9Å resolution

Structure of WRN RQC domain bound to double-stranded DNA

Released:

Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Werner syndrome ATP-dependent helicase Chains: A, B
Molecule details ›
Chains: A, B
Length: 134 amino acids
Theoretical weight: 15.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14191 (Residues: 949-1079; Coverage: 9%)
Gene names: RECQ3, RECQL2, WRN
Sequence domains: RQC domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*AP*CP*CP*CP*TP*AP*AP*TP*TP*AP*GP*GP*GP*T)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 14 nucleotides
Theoretical weight: 4.28 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU, SPRING-8 BEAMLINE BL41XU
Spacegroup: P4322
Unit cell:
a: 84.077Å b: 84.077Å c: 145.291Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.212 0.244
Expression systems:
  • Escherichia coli
  • Not provided