3a9i

X-ray diffraction
1.8Å resolution

Crystal structure of homocitrate synthase from Thermus thermophilus complexed with Lys

Released:

Function and Biology Details

Reaction catalysed:
Acetyl-CoA + H(2)O + 2-oxoglutarate = (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homocitrate synthase Chain: A
Molecule details ›
Chain: A
Length: 376 amino acids
Theoretical weight: 42.25 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: O87198 (Residues: 1-376; Coverage: 100%)
Gene names: TT_C1550, lys20
Sequence domains: HMGL-like
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2221
Unit cell:
a: 80.281Å b: 96.025Å c: 77.082Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.213
Expression system: Escherichia coli