3a9g

X-ray diffraction
2.39Å resolution

Crystal Structure of PQQ-dependent sugar dehydrogenase apo-form

Released:

Function and Biology Details

Reaction catalysed:
D-glucose + ubiquinone = D-glucono-1,5-lactone + ubiquinol
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
GSDH domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 38.37 KDa
Source organism: Pyrobaculum aerophilum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8ZUN8 (Residues: 18-371; Coverage: 95%)
Gene name: PAE2689
Sequence domains: Glucose / Sorbosone dehydrogenase
Structure domains: TolB, C-terminal domain

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: I4122
Unit cell:
a: 177.74Å b: 177.74Å c: 89.937Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.201 0.22
Expression system: Escherichia coli BL21(DE3)