3a8k

X-ray diffraction
1.95Å resolution

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aminomethyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 364 amino acids
Theoretical weight: 40.19 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27248 (Residues: 1-364; Coverage: 100%)
Gene names: JW2873, b2905, gcvT
Sequence domains:
Structure domains:
Glycine cleavage system H protein Chains: E, F
Molecule details ›
Chains: E, F
Length: 129 amino acids
Theoretical weight: 14.01 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6T9 (Residues: 1-129; Coverage: 100%)
Gene names: JW2872, b2904, gcvH
Sequence domains: Glycine cleavage H-protein
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P1
Unit cell:
a: 54.122Å b: 88.412Å c: 97.863Å
α: 91.56° β: 102.42° γ: 89.59°
R-values:
R R work R free
0.174 0.171 0.229
Expression system: Escherichia coli BL21(DE3)