3a8i

X-ray diffraction
1.99Å resolution

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aminomethyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 364 amino acids
Theoretical weight: 40.19 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27248 (Residues: 1-364; Coverage: 100%)
Gene names: JW2873, b2905, gcvT
Sequence domains:
Structure domains:
Glycine cleavage system H protein Chains: E, F
Molecule details ›
Chains: E, F
Length: 129 amino acids
Theoretical weight: 14.01 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6T9 (Residues: 1-129; Coverage: 100%)
Gene names: JW2872, b2904, gcvH
Sequence domains: Glycine cleavage H-protein
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments


Cofactor: Ligand C2F 4 x C2F
1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P1
Unit cell:
a: 53.985Å b: 88.928Å c: 97.993Å
α: 91.53° β: 102.47° γ: 89.59°
R-values:
R R work R free
0.185 0.182 0.244
Expression system: Escherichia coli BL21(DE3)