3a7r

X-ray diffraction
2.05Å resolution

Crystal structure of E. coli lipoate-protein ligase A in complex with lipoyl-AMP.

Released:

Function and Biology Details

Reaction catalysed:
(1a) ATP + (R)-lipoate = lipoyl-AMP + diphosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipoate-protein ligase A Chain: A
Molecule details ›
Chain: A
Length: 337 amino acids
Theoretical weight: 37.84 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P32099 (Residues: 2-338; Coverage: 100%)
Gene names: JW4349, b4386, lplA, yjjF
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P6122
Unit cell:
a: 102.298Å b: 102.298Å c: 221.252Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.176 0.204
Expression system: Escherichia coli