3a5o

X-ray diffraction
2.4Å resolution

Crystal Structure of a Dictyostelium P109I Ca2+-Actin in Complex with Human Gelsolin Segment 1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gelsolin Chain: S
Molecule details ›
Chain: S
Length: 127 amino acids
Theoretical weight: 14.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06396 (Residues: 53-176; Coverage: 16%)
Gene name: GSN
Sequence domains: Gelsolin repeat
Structure domains: Severin
Major actin Chain: C
Molecule details ›
Chain: C
Length: 375 amino acids
Theoretical weight: 41.47 KDa
Source organism: Dictyostelium discoideum
Expression system: Dictyostelium
UniProt:
  • Canonical: P07830 (Residues: 2-376; Coverage: 100%)
Gene names: DDB_G0269234, DDB_G0272248, DDB_G0272520, DDB_G0274129, DDB_G0274133, DDB_G0274135, DDB_G0274137, DDB_G0274285, DDB_G0274561, DDB_G0274599, DDB_G0274601, DDB_G0274727, DDB_G0280545, DDB_G0288879, DDB_G0289005, DDB_G0289553, DDB_G0289663, act1, act11, act12, act13, act14, act15, act16, act19, act1a, act2, act2-1, act20, act21, act4, act5, act6, act7, act8, act9, actA1, actA8, actB1, actM6
Sequence domains: Actin
Structure domains:

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 56.852Å b: 69.005Å c: 180.881Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.207
Expression systems:
  • Escherichia coli
  • Dictyostelium