› 3a3q

X-ray diffraction
2Å resolution

Structure of N59D HEN EGG-WHITE LYSOZYME in complex with (GlcNAc)3

Released:
DOI: 10.2210/pdb3a3q/pdb
PDB entry 3a3q coloured by chain, front view.
PDB entry 3a3q coloured by chain, side view.
PDB entry 3a3q coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Importance of the hydrogen bonding network including Asp52 for catalysis, as revealed by Asn59 mutant hen egg-white lysozymes.
Ose T, Kuroki K, Matsushima M, Maenaka K, Kumagai I
J Biochem 146 651-7 (2009)
PMID: 19605465

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE
Spacegroup: P43212
Unit cell:
a: 78.71Å b: 78.71Å c: 38.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.205 0.287
Expression system: Saccharomyces cerevisiae

Quick links

Citations

9 citation in other articles

Evolution of the genetic code by incorporation of amino acids that improved or changed protein function.
Francis BR. (2013)
8 more

1 mention without citation

Structural, Functional and Phylogenetic Analysis of Sperm Lysozyme-Like Proteins.
Kalra et al. (2016)