› 3a2s

X-ray diffraction
2.2Å resolution

Crystal Structure of outer membrane protein PorB from Neisseria meningitidis in complex with sucrose

Released:
DOI: 10.2210/pdb3a2s/pdb
PDB entry 3a2s coloured by chain, front view.
PDB entry 3a2s coloured by chain, side view.
PDB entry 3a2s coloured by chain, top view.
Source organism: Neisseria meningitidis
Primary publication:
Structural basis for solute transport, nucleotide regulation, and immunological recognition of Neisseria meningitidis PorB.
Tanabe M, Nimigean CM, Iverson TM
Proc Natl Acad Sci U S A 107 6811-6 (2010)
PMID: 20351243

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177788 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Outer membrane protein II Chain: X
Molecule details ›
Chain: X
Length: 355 amino acids
Theoretical weight: 38.18 KDa
Source organism: Neisseria meningitidis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5RT80 (Residues: 1-322; Coverage: 100%)
Gene name: porB
Sequence domains: Gram-negative porin
Structure domains: Porin

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
1 bound ligand:
Ligand LDA 1 x LDA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P63
Unit cell:
a: 82.773Å b: 82.773Å c: 106.433Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.209 0.256
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Intrinsic danger: activation of Toll-like receptors in rheumatoid arthritis.
Goh et al. (2012)
5 more

5 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
4 more