3a0n

X-ray diffraction
1.45Å resolution

Crystal structure of D-glucuronic acid-bound alginate lyase vAL-1 from Chlorella virus

Released:

Function and Biology Details

Reaction catalysed:
Eliminative cleavage of alginate to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing ends and beta-D-mannuronate at their reducing end.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
VAL-1 Chain: A
Molecule details ›
Chain: A
Length: 252 amino acids
Theoretical weight: 28.04 KDa
Source organism: Paramecium bursaria Chlorella virus CVK2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9DTZ2 (Residues: 106-349; Coverage: 70%)
Gene name: vAL-1
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 40.488Å b: 71.046Å c: 99.316Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 not available 0.223
Expression system: Escherichia coli BL21(DE3)