PDBe 3zn8

Electron Microscopy
12Å resolution

Structural Basis of Signal Sequence Surveillance and Selection by the SRP-SR Complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
Dipeptidyl aminopeptidase B Chain: S
Molecule details ›
Chain: S
Length: 14 amino acids
Theoretical weight: 1.52 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P18962 (Residues: 31-44; Coverage: 2%)
Gene names: DAP2, YHR028C
Signal recognition particle protein Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 32.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O07347 (Residues: 2-295; Coverage: 68%)
Gene name: ffh
Sequence domains:
Signal recognition particle receptor FtsY Chain: D
Molecule details ›
Chain: D
Length: 295 amino acids
Theoretical weight: 32.18 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P10121 (Residues: 201-495; Coverage: 59%)
Gene names: JW3429, b3464, ftsY
Sequence domains:
Signal recognition particle 54 kDa protein Chain: M
Molecule details ›
Chain: M
Length: 125 amino acids
Theoretical weight: 14.59 KDa
Source organism: Sulfolobus solfataricus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q97ZE7 (Residues: 308-431; Coverage: 28%)
Gene names: SSO0971, srp54
Sequence domains: Signal peptide binding domain
4.5 S RNA Chain: G
Molecule details ›
Chain: G
Length: 88 nucleotides
Theoretical weight: 28.51 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 12Å
Relevant EMDB volumes: EMD-2316
Expression system: Escherichia coli BL21