PDBe 3zia

X-ray diffraction
2.5Å resolution

The structure of F1-ATPase from Saccharomyces cerevisiae inhibited by its regulatory protein IF1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
ATP synthase subunit alpha, mitochondrial Chains: A, B, C, K, L, M
Molecule details ›
Chains: A, B, C, K, L, M
Length: 510 amino acids
Theoretical weight: 55.01 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P07251 (Residues: 36-545; Coverage: 94%)
Gene names: ATP1, YBL0827, YBL099W
Sequence domains:
Structure domains:
ATP synthase subunit beta, mitochondrial Chains: D, E, F, N, O, P
ATP synthase subunit gamma, mitochondrial Chains: G, Q
Molecule details ›
Chains: G, Q
Length: 278 amino acids
Theoretical weight: 30.66 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38077 (Residues: 34-311; Coverage: 89%)
Gene names: ATP3, ATP3a, ATP3b, YBR039W, YBR0408
Sequence domains: ATP synthase
Structure domains:
ATP synthase subunit delta, mitochondrial Chains: H, R
Molecule details ›
Chains: H, R
Length: 138 amino acids
Theoretical weight: 14.57 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12165 (Residues: 23-160; Coverage: 86%)
Gene names: ATP16, D2935, YD8119.03, YDL004W
Sequence domains: ATP synthase, Delta/Epsilon chain, beta-sandwich domain
ATP synthase subunit epsilon, mitochondrial Chains: I, S
Molecule details ›
Chains: I, S
Length: 61 amino acids
Theoretical weight: 6.62 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P21306 (Residues: 2-62; Coverage: 98%)
Gene names: ATP15, P0345, YPL271W
Sequence domains: Mitochondrial ATP synthase epsilon chain
Structure domains: ATP synthase, F1 complex, epsilon subunit superfamily, mitochondrial
ATPase inhibitor, mitochondrial Chains: J, T
Molecule details ›
Chains: J, T
Length: 63 amino acids
Theoretical weight: 7.34 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01097 (Residues: 23-85; Coverage: 74%)
Gene names: D1305, INH1, YDL181W

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21
Unit cell:
a: 118.224Å b: 187.846Å c: 181.812Å
α: 90° β: 89.96° γ: 90°
R-values:
R R work R free
0.226 0.224 0.262
Expression system: Escherichia coli BL21(DE3)