PDBe 3zf4

X-ray diffraction
3.1Å resolution

Phage dUTPases control transfer of virulence genes by a proto- oncogenic G protein-like mechanism. (Staphylococcus bacteriophage 80alpha dUTPase Y81A mutant with dUpNHpp).

Released:

Function and Biology Details

Reaction catalysed:
dUTP + H(2)O = dUMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dUTPase Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 22.58 KDa
Source organism: Staphylococcus virus 80alpha
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4ZF98 (Residues: 1-170; Coverage: 100%)
Sequence domains: dUTPase
Structure domains: Deoxyuridine 5'-Triphosphate Nucleotidohydrolase; Chain A

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P213
Unit cell:
a: 87.47Å b: 87.47Å c: 87.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.24 0.291
Expression system: Escherichia coli BL21(DE3)