X-ray diffraction
1.47Å resolution

Structure of E. coli ExoIX in complex with the palindromic 5ov4 DNA oligonucleotide, di-magnesium and potassium


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Flap endonuclease Xni Chain: A
Molecule details ›
Chain: A
Length: 251 amino acids
Theoretical weight: 28.2 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q8X6R9 (Residues: 1-251; Coverage: 100%)
Gene names: ECs3658, Z4115, xni, ygdG
Sequence domains:
Structure domains:
5OV4 DNA, 5'-D(*AP*AP*AP*AP*GP*CP*GP*TP*AP*CP*GP*CP)-3' Chain: X
Molecule details ›
Chain: X
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21212
Unit cell:
a: 66.238Å b: 152.302Å c: 34.522Å
α: 90° β: 90° γ: 90°
R R work R free
0.204 0.203 0.231
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided