3wof

X-ray diffraction
3.3Å resolution

Crystal structure of P23-45 gp39 (6-132) bound to Thermus thermophilus RNA polymerase beta-flap domain

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA-directed RNA polymerase subunit beta Chains: A, C, E, G, I, K, M, O, Q, S, U, W
Molecule details ›
Chains: A, C, E, G, I, K, M, O, Q, S, U, W
Length: 130 amino acids
Theoretical weight: 14.48 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RQE9 (Residues: 703-830; Coverage: 11%)
Gene names: TTHA1813, rpoB
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Putative uncharacterized protein Chains: B, D, F, H, J, L, N, P, R, T, V, X
Molecule details ›
Chains: B, D, F, H, J, L, N, P, R, T, V, X
Length: 129 amino acids
Theoretical weight: 14.85 KDa
Source organism: Thermus virus P23-45
Expression system: Escherichia coli
UniProt:
  • Canonical: A7XX65 (Residues: 6-132; Coverage: 90%)
Gene name: P23p39
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P41212
Unit cell:
a: 213.777Å b: 213.777Å c: 234.598Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.219 0.246
Expression system: Escherichia coli