3wnx

X-ray diffraction
2.75Å resolution

Crystal structure of ERGIC-53/MCFD2, Calcium/Man3-bound form

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein ERGIC-53 Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 27.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49257 (Residues: 31-269; Coverage: 50%)
Gene names: ERGIC53, F5F8D, LMAN1
Sequence domains: Legume-like lectin family
Structure domains: Jelly Rolls
Multiple coagulation factor deficiency protein 2 Chain: B
Molecule details ›
Chain: B
Length: 104 amino acids
Theoretical weight: 12.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NI22 (Residues: 67-146; Coverage: 67%)
Gene names: MCFD2, SDNSF
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2
Unit cell:
a: 100.257Å b: 58.212Å c: 55.435Å
α: 90° β: 109.32° γ: 90°
R-values:
R R work R free
0.206 0.203 0.287
Expression system: Escherichia coli