PDBe 3wks

X-ray diffraction
3.03Å resolution

Crystal structure of the SepCysS-SepCysE N-terminal domain complex from

Released:

Function and Biology Details

Reaction catalysed:
O-phospho-L-seryl-tRNA(Cys) + sulfide = L-cysteinyl-tRNA(Cys) + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Uncharacterized protein MJ1481 Chains: C, D
Molecule details ›
Chains: C, D
Length: 106 amino acids
Theoretical weight: 12.55 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58876 (Residues: 1-103; Coverage: 48%)
Gene name: MJ1481
O-phospho-L-seryl-tRNA:Cys-tRNA synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 416 amino acids
Theoretical weight: 47.42 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59072 (Residues: 2-377; Coverage: 100%)
Gene name: MJ1678
Sequence domains: O-phosphoseryl-tRNA(Sec) selenium transferase, SepSecS
Structure domains:

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P212121
Unit cell:
a: 95.073Å b: 107.135Å c: 110.762Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.189 0.24
Expression system: Escherichia coli