PDBe 3wkr

X-ray diffraction
2.8Å resolution

Crystal structure of the SepCysS-SepCysE complex from Methanocaldococcus jannaschii

Released:

Function and Biology Details

Reaction catalysed:
O-phospho-L-seryl-tRNA(Cys) + sulfide = L-cysteinyl-tRNA(Cys) + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Uncharacterized protein MJ1481 Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 216 amino acids
Theoretical weight: 25.33 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58876 (Residues: 1-213; Coverage: 100%)
Gene name: MJ1481
Sequence domains: Uncharacterized protein conserved in archaea (DUF2100)
O-phospho-L-seryl-tRNA:Cys-tRNA synthase Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 416 amino acids
Theoretical weight: 47.42 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59072 (Residues: 2-377; Coverage: 100%)
Gene name: MJ1678
Sequence domains: O-phosphoseryl-tRNA(Sec) selenium transferase, SepSecS
Structure domains:

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P65
Unit cell:
a: 106.88Å b: 106.88Å c: 495.253Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.224 0.242
Expression system: Escherichia coli