3wkn

X-ray diffraction
2.9Å resolution

Crystal structure of the artificial protein AFFinger p17 (AF.p17) complexed with Fc fragment of human IgG

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin heavy constant gamma 1 Chains: A, B, C, D, I, J, M, N
Molecule details ›
Chains: A, B, C, D, I, J, M, N
Length: 212 amino acids
Theoretical weight: 23.98 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P01857 (Residues: 119-330; Coverage: 64%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
AFFinger p17 Chains: E, F, G, H, K, L, O, P
Molecule details ›
Chains: E, F, G, H, K, L, O, P
Length: 54 amino acids
Theoretical weight: 6.12 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: N-terminal domain of TfIIb

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL, FUC
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 104.027Å b: 153.428Å c: 170.132Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.242
Expression systems:
  • Cricetulus griseus
  • Escherichia coli BL21(DE3)