› 3wju

X-ray diffraction
2.5Å resolution

Crystal structure of the L68D variant of mLolB from Escherichia coli

Released:
DOI: 10.2210/pdb3wju/pdb
PDB entry 3wju coloured by chain, front view.
PDB entry 3wju coloured by chain, side view.
PDB entry 3wju coloured by chain, top view.
Source organism: Escherichia coli DH1
Primary publication:
Roles of the protruding loop of factor B essential for the localization of lipoproteins (LolB) in the anchoring of bacterial triacylated proteins to the outer membrane.
Hayashi Y, Tsurumizu R, Tsukahara J, Takeda K, Narita SI, Mori M, Miki K, Tokuda H
J Biol Chem 289 10530-10539 (2014)
PMID: 24569999

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164220 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer-membrane lipoprotein LolB Chain: A
Molecule details ›
Chain: A
Length: 178 amino acids
Theoretical weight: 20.48 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli
Structure domains: Lipoprotein localisation LolA/LolB/LppX

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P6522
Unit cell:
a: 100.889Å b: 100.889Å c: 97.092Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.249 0.249 0.291
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Lipid trafficking across the Gram-negative cell envelope.
Shrivastava et al. (2019)
6 other articles

1 mention without citation

Roles of the protruding loop of factor B essential for the localization of lipoproteins (LolB) in the anchoring of bacterial triacylated proteins to the outer membrane.
Hayashi et al. (2014)