› 3wah

X-ray diffraction
1.54Å resolution

Crystal Structure of HasAp with Iron MesoporphyrinIX

Released:
DOI: 10.2210/pdb3wah/pdb
PDB entry 3wah coloured by chain, front view.
PDB entry 3wah coloured by chain, side view.
PDB entry 3wah coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Inhibition of heme uptake in Pseudomonas aeruginosa by its hemophore (HasA(p)) bound to synthetic metal complexes.
Shirataki C, Shoji O, Terada M, Ozaki S, Sugimoto H, Shiro Y, Watanabe Y
Angew Chem Int Ed Engl 53 2862-6 (2014)
PMID: 24604808

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124397 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme acquisition protein HasAp Chains: A, B
Molecule details ›
Chains: A, B
Length: 186 amino acids
Theoretical weight: 19.05 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: G3XD33 (Residues: 1-184; Coverage: 90%)
Gene names: PA3407, hasAp
Sequence domains: Heme-binding protein A (HasA)
Structure domains: Haem-binding HasA

Ligands and Environments

1 bound ligand:
Ligand MH0 2 x MH0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P31
Unit cell:
a: 47.518Å b: 47.518Å c: 140.893Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.175 0.194
Expression system: Escherichia coli

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Citations

3 review citations

Extracellular Heme Uptake and the Challenge of Bacterial Cell Membranes.
Huang et al. (2017)
2 more