3w6v

X-ray diffraction
2.95Å resolution

Crystal structure of the DNA-binding domain of AdpA, the global transcriptional factor, in complex with a target DNA

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH araC/xylS-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 147 amino acids
Theoretical weight: 16.74 KDa
Source organism: Streptomyces griseus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9S166 (Residues: 215-340; Coverage: 31%)
Gene names: NCTC13033_02525, adpA, rhaS_1
Sequence domains: Helix-turn-helix domain
Structure domains: Homeodomain-like
DNA (5'-D(*CP*TP*GP*TP*GP*AP*AP*CP*CP*CP*GP*CP*CP*AP*AP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.83 KDa
Source organism: Streptomyces griseus
Expression system: Not provided
DNA (5'-D(*AP*GP*GP*TP*TP*GP*GP*CP*GP*GP*GP*TP*TP*CP*AP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.97 KDa
Source organism: Streptomyces griseus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: C2221
Unit cell:
a: 76.51Å b: 100.64Å c: 100.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.236 0.278
Expression systems:
  • Escherichia coli
  • Not provided