PDBe 3w3d

X-ray diffraction
1.8Å resolution

Crystal structure of smooth muscle G actin DNase I complex

Released:
Source organisms:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, gamma-enteric smooth muscle Chain: A
Molecule details ›
Chain: A
Length: 374 amino acids
Theoretical weight: 41.73 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P63270 (Residues: 3-376; Coverage: 100%)
Gene names: ACTA3, ACTG2, ACTSG
Sequence domains: Actin
Structure domains:
Deoxyribonuclease-1 Chain: B
Molecule details ›
Chain: B
Length: 260 amino acids
Theoretical weight: 29.09 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00639 (Residues: 23-282; Coverage: 100%)
Gene names: DNASE1, DNL1
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 42Å b: 225.3Å c: 77.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 not available