X-ray diffraction
3Å resolution

Crystal structure of Saccharomyces cerevisiae Atg8 complexed with Atg32 AIM


Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Autophagy-related protein 8 Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 13.76 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
  • Canonical: P38182 (Residues: 1-116; Coverage: 99%)
Gene names: APG8, ATG8, AUT7, CVT5, YBL0732, YBL078C
Sequence domains: Autophagy protein Atg8 ubiquitin like
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Autophagy-related protein 32 Chain: B
Molecule details ›
Chain: B
Length: 6 amino acids
Theoretical weight: 732 Da
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
  • Canonical: P40458 (Residues: 85-90; Coverage: 1%)
Gene names: ATG32, ECM17, YIL146C

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: I23
Unit cell:
a: 104.836Å b: 104.836Å c: 104.836Å
α: 90° β: 90° γ: 90°
R R work R free
0.228 0.223 0.272
Expression systems:
  • Escherichia coli
  • Not provided