X-ray diffraction
1.98Å resolution

Crystal structures of D-Psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars


Function and Biology Details

Reaction catalysed:
D-psicose = D-fructose
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
D-psicose 3-epimerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 294 amino acids
Theoretical weight: 33.15 KDa
Source organism: Ruminiclostridium cellulolyticum H10
Expression system: Escherichia coli
  • Canonical: B8I944 (Residues: 1-293; Coverage: 100%)
Gene name: Ccel_0941
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P21
Unit cell:
a: 79.761Å b: 115.441Å c: 91.627Å
α: 90° β: 105.45° γ: 90°
R R work R free
0.175 0.175 0.22
Expression system: Escherichia coli