PDBe 3vag

X-ray diffraction
2.19Å resolution

Structure of U2AF65 variant with BrU3C2 DNA

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Splicing factor U2AF 65 kDa subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 174 amino acids
Theoretical weight: 19.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Best match: P26368 (Residues: 148-336)
Gene names: U2AF2, U2AF65
DNA 5'-D(*U*CP*(BRU)P*UP*UP*UP*U)-3' Chains: E, P
Molecule details ›
Chains: E, P
Length: 7 nucleotides
Theoretical weight: 2.06 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2
Unit cell:
a: 166.825Å b: 37.499Å c: 100.925Å
α: 90° β: 125.35° γ: 90°
R-values:
R R work R free
0.195 0.191 0.232
Expression systems:
  • Escherichia coli
  • Not provided