PDBe 3v61

X-ray diffraction
2.8Å resolution

Structure of S. cerevisiae PCNA conjugated to SUMO on lysine 164

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin-like protein SMT3 Chain: A
Molecule details ›
Chain: A
Length: 84 amino acids
Theoretical weight: 9.72 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12306 (Residues: 20-98; Coverage: 78%)
Gene names: D9719.15, SMT3, YDR510W
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Proliferating cell nuclear antigen Chain: B
Molecule details ›
Chain: B
Length: 258 amino acids
Theoretical weight: 28.87 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15873 (Residues: 1-258; Coverage: 100%)
Gene names: POL30, YBR0811, YBR088C
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I41
Unit cell:
a: 140.06Å b: 140.06Å c: 52.119Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.254
Expression system: Escherichia coli BL21(DE3)