PDBe 3uw4

X-ray diffraction
1.79Å resolution

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Baculoviral IAP repeat-containing protein 2; E3 ubiquitin-protein ligase XIAP Chain: A
Molecule details ›
Chain: A
Length: 92 amino acids
Theoretical weight: 10.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13490 (Residues: 266-331, 334-343; Coverage: 12%)
  • Canonical: P98170 (Residues: 326-327, 338-348; Coverage: 3%)
Gene names: API1, API3, BIRC2, BIRC4, IAP3, MIHB, RNF48, XIAP
Sequence domains: Inhibitor of Apoptosis domain
Structure domains: Inhibitor Of Apoptosis Protein (2mihbC-IAP-1); Chain A
GDC0152 Chain: Z
Molecule details ›
Chain: Z
Length: 4 amino acids
Theoretical weight: 499 Da

Ligands and Environments

1 bound ligand:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 35.691Å b: 37.38Å c: 57.598Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.177 0.221
Expression system: Escherichia coli