3uvk

X-ray diffraction
1.4Å resolution

Crystal structure of WDR5 in complex with the WDR5-interacting motif of MLL2

Released:

Function and Biology Details

Reaction catalysed:
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(4) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(4)

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
WD repeat-containing protein 5 Chain: A
Molecule details ›
Chain: A
Length: 318 amino acids
Theoretical weight: 34.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61964 (Residues: 21-334; Coverage: 94%)
Gene names: BIG3, WDR5
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Histone-lysine N-methyltransferase 2D Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.18 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O14686 (Residues: 5337-5347; Coverage: 0%)
Gene names: ALR, KMT2D, MLL2, MLL4

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 48.827Å b: 68.496Å c: 87.815Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.151 0.192
Expression systems:
  • Escherichia coli
  • Not provided