3uux

X-ray diffraction
3.9Å resolution

Crystal structure of yeast Fis1 in complex with Mdv1 fragment containing N-terminal extension and coiled coil domains

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitochondrial fission 1 protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 129 amino acids
Theoretical weight: 15.13 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P40515 (Residues: 1-129; Coverage: 83%)
Gene names: FIS1, MDV2, YIL065C
Sequence domains:
Structure domains: Tetratricopeptide repeat domain
Mitochondrial division protein 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 242 amino acids
Theoretical weight: 27.14 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P47025 (Residues: 94-314; Coverage: 31%)
Gene names: FIS2, GAG3, J0802, MDV1, NET2, YJL112W
Sequence domains: Mitochondrial division protein 1
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: I422
Unit cell:
a: 174.676Å b: 174.676Å c: 167.301Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.271 0.27 0.284
Expression system: Escherichia coli