› 3uni

X-ray diffraction
2.2Å resolution

Crystal Structure of Bovine Milk Xanthine Dehydrogenase with NADH Bound

Released:
DOI: 10.2210/pdb3uni/pdb
PDB entry 3uni coloured by chain, front view.
PDB entry 3uni coloured by chain, side view.
PDB entry 3uni coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Protein conformational gating of enzymatic activity in xanthine oxidoreductase.
Ishikita H, Eger BT, Okamoto K, Nishino T, Pai EF
J Am Chem Soc 134 999-1009 (2012)
PMID: 22145797

Function and Biology Details

Reactions catalysed: 
Xanthine + NAD(+) + H(2)O = urate + NADH
Xanthine + H(2)O + O(2) = urate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD

Cofactor: Ligand MTE 2 x MTE

Cofactor: Ligand NAI 2 x NAI
6 bound ligands:
Ligand FES 4 x FES
Ligand MOS 2 x MOS
Ligand GOL 9 x GOL
Ligand CO3 2 x CO3
Ligand SAL 2 x SAL
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P21
Unit cell:
a: 100.036Å b: 146.698Å c: 107.016Å
α: 90° β: 106.03° γ: 90°
R-values:
R R work R free
0.198 0.198 0.24

Quick links

Citations

7 review citations

Proton transfer reactions and hydrogen-bond networks in protein environments.
Ishikita et al. (2014)
6 more

4 mentions without citation

Protein conformational gating of enzymatic activity in xanthine oxidoreductase.
Ishikita et al. (2012)
3 more